TIME-LAPSE VARIABILITY OF HUMAN EMBRYOS IS ASSOCIATED WITH IVF OUTCOME

Many morphokinetic parameters obtained from human embryos cultured in time-lapse imaging systems have been evaluated as biomarkers for optimal selection of with high implantation potential. However, it is not clear whether the variability among same cycle differ between cases successful and unsuccessful implantation. The purpose this study was to quantify compare women 1712 blastocysts generated ICSI January 2017 November 2020, derived 437 patients were included retrospective study. Among them, 170 had (positive beta hCG test) 267 (negative after embryo transfer studied group. Only more than 2 per All single culture medium (Life Global, Brussels, Belgium) a system (EmbryoScope, Vitrolife, Sweden). Fifteen analyzed: time pronuclei appearance (tPNa), fading (tPNf), cleavage times (t2, t3, t4, t5, t6, t7, t8, t9), morulae formation (tM), starting blastulation (tSB), full blastocyst stage (tB), expansion (tEB) hatching timing (tHB). Statistical analysis performed by IBM SPSS Software v.21. Inter-embryo each woman/cycle measured coefficient variation (CV), expressed percentage. CV value patient compared without using Student t-test. P<0.05 considered statistically significant. There no significant differences age, 3 Day FSH, BMI morphological quality groups (p>0.05, t-test). there difference values 8 variables – tPNa (4% vs. 8%, respectively, P=0.001), t3 (6% 9%, P=0.021), t4 (5% P=0.004), t6 P=0.012), t7 P=0.005), tM 7%, P=0.010), tSB (1% 2%, P=0.033), tB (0.7% 1%, P=0.024). Lower inter-embryo associated higher chance could be potential predictor